Insulin-like growth factor system components expressed at the conceptus-maternal interface during the establishment of equine pregnancy (2024)

Gibson, C., de Ruijter-Villani, M. (2022). Insulin-like growth factor system components expressed at the conceptus-maternal interface during the establishment of equine pregnancy. Frontiers in Veterinary Science, 9, 1-15. Article 912721. https://doi.org/10.3389/fvets.2022.912721

Gibson, Charlotte ; de Ruijter-Villani, M ; Stout, Tom A E. / Insulin-like growth factor system components expressed at the conceptus-maternal interface during the establishment of equine pregnancy. In: Frontiers in Veterinary Science. 2022 ; Vol. 9. pp. 1-15.

@article{48dd54f70a15434b9cce7866fcec0c4c,

title = "Insulin-like growth factor system components expressed at the conceptus-maternal interface during the establishment of equine pregnancy",

abstract = "In many species, the insulin-like growth factors (IGF1 and IGF2), their receptors and IGF binding proteins play important roles in preparing the endometrium for implantation, and regulating conceptus growth and development. To determine whether the IGF system may contribute to conceptus-maternal interaction during equine pre-implantation development, we evaluated mRNA expression for IGF system components in conceptuses, and endometrium recovered from pregnant and cycling mares, on days 7, 14, 21 and 28 after ovulation. We also investigated expression of IGF1, IGF2 and their receptors 6 and 11 days after transfer of day 8 embryos to synchronous (day 8) or asynchronous (day 3) recipient mares. Expression of IGF1 and IGF2, IGF1R, IGF2R, INSR and IGFBPs 1, 2, 4 and 5 was evident in endometrium and conceptus membranes during days 7-28. Endometrial IGF2, INSR, IGFBP1 and IGFBP2 expression increased between days 7 and 28 of pregnancy. In conceptus membranes, expression of all IGF system components increased with developmental stage. Immunohistochemistry revealed strong expression of IGF1, IGF2 and IGF1R in both endometrium and conceptus membranes, whereas INSR was highly expressed in endometrium but barely detectable in the conceptus. Finally, a negatively asynchronous uterine environment retarded IGF1, IGF2 and INSR expression in the conceptus, whereas in the endometrium only INSR expression was altered by asynchrony. The presence of IGFs, their receptors and IGFBPs in the endometrium and conceptus during early equine pregnancy, and down-regulation in the conceptus following asynchronous embryo transfer, suggest a role in conceptus-maternal communication during the preparation for implantation. ",

keywords = "asynchronous embryo transfer, conceptus, endometrium, equine, insulin-like growth factor, pregnancy",

author = "Charlotte Gibson and {de Ruijter-Villani}, M and Stout, {Tom A E}",

note = "Funding Information: This research was supported by the European Commission under the Marie Curie ITN project EpiHealthNet; FP7-PEOPLE-2012-ITN n° 317146. Funding Information: The authors would like to thank Andras Dinnyes, the scientific co-ordinator of EpiHealthNet. Publisher Copyright: Copyright {\textcopyright} 2022 Gibson, de Ruijter-Villani and Stout.",

year = "2022",

month = sep,

day = "13",

doi = "10.3389/fvets.2022.912721",

language = "English",

volume = "9",

pages = "1--15",

journal = "Frontiers in Veterinary Science",

issn = "2297-1769",

publisher = "Frontiers Media S.A.",

}

Gibson, C, de Ruijter-Villani, M 2022, 'Insulin-like growth factor system components expressed at the conceptus-maternal interface during the establishment of equine pregnancy', Frontiers in Veterinary Science, vol. 9, 912721, pp. 1-15. https://doi.org/10.3389/fvets.2022.912721

Insulin-like growth factor system components expressed at the conceptus-maternal interface during the establishment of equine pregnancy. / Gibson, Charlotte; de Ruijter-Villani, M; Stout, Tom A E.
In: Frontiers in Veterinary Science, Vol. 9, 912721, 13.09.2022, p. 1-15.

Research output: Contribution to journal › Article › Academic › peer-review

TY - JOUR

T1 - Insulin-like growth factor system components expressed at the conceptus-maternal interface during the establishment of equine pregnancy

AU - Gibson, Charlotte

AU - de Ruijter-Villani, M

AU - Stout, Tom A E

N1 - Funding Information:This research was supported by the European Commission under the Marie Curie ITN project EpiHealthNet; FP7-PEOPLE-2012-ITN n° 317146.Funding Information:The authors would like to thank Andras Dinnyes, the scientific co-ordinator of EpiHealthNet.Publisher Copyright:Copyright © 2022 Gibson, de Ruijter-Villani and Stout.

PY - 2022/9/13

Y1 - 2022/9/13

N2 - In many species, the insulin-like growth factors (IGF1 and IGF2), their receptors and IGF binding proteins play important roles in preparing the endometrium for implantation, and regulating conceptus growth and development. To determine whether the IGF system may contribute to conceptus-maternal interaction during equine pre-implantation development, we evaluated mRNA expression for IGF system components in conceptuses, and endometrium recovered from pregnant and cycling mares, on days 7, 14, 21 and 28 after ovulation. We also investigated expression of IGF1, IGF2 and their receptors 6 and 11 days after transfer of day 8 embryos to synchronous (day 8) or asynchronous (day 3) recipient mares. Expression of IGF1 and IGF2, IGF1R, IGF2R, INSR and IGFBPs 1, 2, 4 and 5 was evident in endometrium and conceptus membranes during days 7-28. Endometrial IGF2, INSR, IGFBP1 and IGFBP2 expression increased between days 7 and 28 of pregnancy. In conceptus membranes, expression of all IGF system components increased with developmental stage. Immunohistochemistry revealed strong expression of IGF1, IGF2 and IGF1R in both endometrium and conceptus membranes, whereas INSR was highly expressed in endometrium but barely detectable in the conceptus. Finally, a negatively asynchronous uterine environment retarded IGF1, IGF2 and INSR expression in the conceptus, whereas in the endometrium only INSR expression was altered by asynchrony. The presence of IGFs, their receptors and IGFBPs in the endometrium and conceptus during early equine pregnancy, and down-regulation in the conceptus following asynchronous embryo transfer, suggest a role in conceptus-maternal communication during the preparation for implantation.

AB - In many species, the insulin-like growth factors (IGF1 and IGF2), their receptors and IGF binding proteins play important roles in preparing the endometrium for implantation, and regulating conceptus growth and development. To determine whether the IGF system may contribute to conceptus-maternal interaction during equine pre-implantation development, we evaluated mRNA expression for IGF system components in conceptuses, and endometrium recovered from pregnant and cycling mares, on days 7, 14, 21 and 28 after ovulation. We also investigated expression of IGF1, IGF2 and their receptors 6 and 11 days after transfer of day 8 embryos to synchronous (day 8) or asynchronous (day 3) recipient mares. Expression of IGF1 and IGF2, IGF1R, IGF2R, INSR and IGFBPs 1, 2, 4 and 5 was evident in endometrium and conceptus membranes during days 7-28. Endometrial IGF2, INSR, IGFBP1 and IGFBP2 expression increased between days 7 and 28 of pregnancy. In conceptus membranes, expression of all IGF system components increased with developmental stage. Immunohistochemistry revealed strong expression of IGF1, IGF2 and IGF1R in both endometrium and conceptus membranes, whereas INSR was highly expressed in endometrium but barely detectable in the conceptus. Finally, a negatively asynchronous uterine environment retarded IGF1, IGF2 and INSR expression in the conceptus, whereas in the endometrium only INSR expression was altered by asynchrony. The presence of IGFs, their receptors and IGFBPs in the endometrium and conceptus during early equine pregnancy, and down-regulation in the conceptus following asynchronous embryo transfer, suggest a role in conceptus-maternal communication during the preparation for implantation.

KW - asynchronous embryo transfer

KW - conceptus

KW - endometrium

KW - equine

KW - insulin-like growth factor

KW - pregnancy

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U2 - 10.3389/fvets.2022.912721

DO - 10.3389/fvets.2022.912721

M3 - Article

C2 - 36176700

SN - 2297-1769

VL - 9

SP - 1

EP - 15

JO - Frontiers in Veterinary Science

JF - Frontiers in Veterinary Science

M1 - 912721

ER -

Gibson C, de Ruijter-Villani M, Stout TAE. Insulin-like growth factor system components expressed at the conceptus-maternal interface during the establishment of equine pregnancy. Frontiers in Veterinary Science. 2022 Sept 13;9:1-15. 912721. doi: 10.3389/fvets.2022.912721